The Protein Function Score (PFS) server provides for a quick evaluation of
Swiss-Prot entries with particular regard to protein function.
The value of PFS for a given protein is estimated by
scanning the entry information for the number and quality
of publications, protein descriptions and comments
such as FUNCTION and CATALYTIC ACTIVITY. The resulting number between
zero and three reflects the current information content of every given
Swiss-Prot member, ascending with the degree of annotation.
The manual inspection of Blast search data is a laborious task with
the goal to find the best hit delivering the most informative
content. By including color codes to Blast search results visualizing
the three different PFS levels, hits are secondarily ranked according
to their knowledge contribution and therefore more easy to analyse.
Our PFS webservice provides the following utilities:
Result lists may be sorted alphabetically or numerically by a mouse click
in the column header. Accession numbers in the output tables provide
a link to respective Swiss-Prot entries at www.uniprot.org.
- The retrieval of PFS for any given Swiss-Prot entry
specified by an entry name, accession number or a keyword
searched in the entry decription and species name.
New: entries now can be filtered by taxon.
A blastp search to compare a query protein against
the Swiss-Prot sequence database. Blast hits are ranked
according to E-value as usual. The PFS values of the
matched proteins are highlighted by color codes with
red for weak annotation, yellow for a moderate and green
for a well characterized entry.
The E-value includes a link to the detailed alignment.